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Nitrofuran (AOZ) rapid test strip

  • Tag:Nitrofuran (AOZ) rapid test strip, fish, shrimp, drug residues
  • Supplier - Shenzhen biokau sci-tech co.,ltd
  • China (Mainland)China (Mainland)
  • US$1 Million - US$2.5 Million
  • 11 - 50 People
  • Manufacturer,Trading Company,Other
  • North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe
  • Ms.Duan
  • 86-0-13724287324
Post Date : January 12
Product Details
Company Profile

Item specifics

Specifications

1. Brief

This product is used for testing Nitrofuran AOZ residue in chicken, aquatic products (fish, shrimp) sample. It is easy to operate, high sensitivity.

2. Detection limit

Tissue: 1ng/g (1ppb)

3. Specifications: 50 tests/kit

4. Principle

The Nitrofuran AOZ rapid test strip is an immuno-chromatographic test, using colloidal gold immunoassay method. It relies on the competition between Nitrofuran AOZ residues in the sample and the Nitrofuran AOZ on T line, to the limited amount of dye-antibody. As a sufficient amount of drug in the sample is presenting, the drug will saturate the antibody. Consequently, it will show an extremely light or even invisible T line, indicating a positive result. On the other hand, if there is a negative sample (or the amount of the drug is lower than the minimum detectable concentration), it will generate two obvious lines in both the T and C line section.

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Anti-pseudorabies virus antibody rapid test card

Anti-pseudorabies virus antibody rapid test card

Anti-pseudorabies virus antibody rapid test card

Ø Principle

This rapid test is an immuno-chromatographic test, using colloidal gold immunoassay method, to detect the antibody against porcine pseudorabies virus in whole blood, serum and plasma.

Ø Test procedure

1) Add 2 drops serum or whole blood sample into the well on the test card;

2) Place the test card on the desktop flatly, incubate for 5-20 minute, then judge the result.

1) Positive: control line and test line are both seen wine red, the more the antibody exist, the thicker the color appear;

2) Negative: only control line is seen wine red;

3) Invalidation: control line isn’t seen wine red.

Ø Consults for diagnosis

1. If not inoculated by the bacterin against porcine pseudorabies virus:

a. test line don’t appear visible wine red show there are no antibodies against porcine pseudorabies virus in tested samples, if the swinery are healthy, it is better to vaccinate in time;

b. test line appear visible wine red show the pig was infected with wild virus, should be more observed to validate.

2. if have been inoculated by the bacterin against porcine pseudorabies virus:

a. the color of test line is thicker than the color of the titer 1:40 from comparison card show the high titer of the antibody against porcine pseudorabies virus, which is enough to resist the strong virus;

Detail more >>
Bird Flu (H5N1) antibody ELISA test kit

Bird Flu (H5N1) antibody ELISA test kit

Bird Flu (H5N1) antibody ELISA test kit

1. Brief

Bird flu (Avian influenza, AI) is an acute contact venereal toxicity infectious disease against the domestic poultry industry at present. Vaccination is the most effective way to prevent and control it, the antibody level after immune reflects the vaccine effect, which directly related to immune resistance to avian influenza virus in chicken flocks.

In Breeders’ immunization work, the most noteworthy is mass immunization vaccination timing issues. In the time between the two rounds of immunization, antibody levels of breeders gradually decreased over time, and at what level to immunization is a headache problem. As at high antibody levels to be immune, it is not only a waste of vaccines, increased economic costs, and high levels of antibodies and vaccines, affect the immune effects of vaccines, lead to immune failure; but immune at the lower levels of antibodies, antibody protection vacuum occurs, threaten the health of breeders.

This product is applicable to the different species, different age in chicken serum specific antibody detection of avian influenza virus. It can be used for avian influenza (virus) vaccine immune time of analysis, evaluation of immune effect, chickens with avian influenza in immune status.

2. Principle

This kit is composed by H5 subtype coated microplate, Enzyme conjugate etc. To detect H5 subtype virus IgG antibodies in chicken serum or plasma by principle of enzyme immunoassay indirect method (ELISA).

4. Components

1

H5 antigen coated microplate

96T×1

2

Enzyme conjugate

11ml×1

3

Sample diluent solution

20ml×2

4

Substrate A

7 ml×1

5

Substrate B

7 ml×1

6

Washing solution (Dilute at 1:25 before use)

10 ml×2

7

Positive control serum

0.7 ml×1

8

Negative control serum

0.7 ml×1

9

Stop solution

7 ml×1

10

Adhesive Foil

3

11

Sealed bag

1 piece

12

Instruction

1 piece

5. Test procedure

1) Return the kit to room temperature for 30mins before use.

2) Take the needed quantity microplate well, set 2 wells of blank control well, 2 wells of negative control well, 2 wells of positive control well, seal the unused plate, store at 2~8°C.

3) Add Negative control serum to negative control well, 100μl/well, Positive control serum to positive control well, 100μl/well; for sample well, dilute sample with sample diluent solution at 200 at first, then add 100μl/well; Add nothing for the blank control well.

4) Mix evenly, Incubation at 37 °C for 30 minutes.

5) Discard liquid of the wells and fill all wells with washing solution, incubate for 60s and discard. Repeat washing procedure 5 times as above, pat to dry.

6) Add enzyme conjugate to each well, 100μl/well.(Except blank control well)

7) Incubation at 37 °C for 30 minutes.

8) Wash as step 5).

9) Add Substrate A 50μl/well, then Substrate B 50μl/well, mix evenly, incubation at 37 °C in dark for 10 minutes.

10) Add stop solution 50μl/well, mix evenly, use ELISA Reader to measure A value at 450nm of each well (Recommend to use ELISA Reader at double-wave 450nm,630nm)

6. Results

1) Negative control well: In normal, A value of negative control well 0.1;

2) Positive control well: In normal, A value of positive control well 0.6;

3) Result judge:

When Sample A450 ≥0.45, that is hemagglutination titer ≥ 6log2, it is well protected;

0.4>A4500.3, the hemagglutination titer is 4log2, it has certain protected power;

When A4500.4, recommend to increase the immune.( A450 is about 1.0 when it is 10 hemagglutination titer)

7. Notes

1) Wear gloves and work clothes when operate, strictly sound and perform disinfection and isolation system.

2) Determination of the test results must be based on ELISA reader.

3) Use liquid adding device to add sample diluent solution and often proof of its accuracy.

4) Add Washing solution to each well fully, to prevent orifice free enzyme, which can not be washed.

5) All the sample washing solution and various waste should be treated as infectious materials.

6) Microplate removed from the refrigerated environment should be balance to dry at room temperature, and seal the unused microplate with desiccant.

7) The test sample should be fresh.

8) Remaining samples and waste shall be approved by the 121 ° C high-pressure steam sterilization for 30 minutes, or deal with 5.0g / L sodium hypochlorite disinfectant for 30 minutes, then abandon.

9) Never mix reagents from different batches.

Specifications: 96 wells/kit.

Expiry date: 12 months.

Storage: Storing at 2-8°C, in the dark.

Detail more >>
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