Nitroimidazole ELISA Test Kit United States Agricultural Waste

Nitroimidazole ELISA Test Kit

Brand Name: REAGEN
Place of Origin: United States
Related Products:REAGEN Nitroimidazole ELISA Test Kit ELSIA KITS KIT

Specifications

Product Description

REAGEN™Nitroimidazole ELISA Test Kit provides a competitive enzyme immunoassay for the quantitative analysis of Nitroimidazole in feed, meat (beef, chicken and pork), eggs, honey.

The unique features of the kit are:

High sensitivity.
A quick ELISA assay (less than 2 hours regardless of number of samples).

High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The drug of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN™Nitroimidazole ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C*. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Nitroimidazole -coated Microtiter Plate	1 x 96-well plate (8 wells x 12 strips)	2-8°C
Nitroimidazole Standards: Negative control 0.1ng/mL 0.3 ng/mL 0.9 ng/mL 2.7 ng/mL 8.1 ng/mL 100ng/mL (spiking, optional)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C
Antibody #1	7 mL	2-8°C *
HRP-Conjugated Antibody #2	12 mL	2-8°C *
TMB Substrate A	7mL	2-8°C
TMB Substrate B	7mL	2-8°C
20X Wash Solution **	40 mL	2-8°C
Stop Buffer **	7 mL	2-8°C
Duplicate dissolve solution	50mL	2-8°C

* If you are not planning to use the kit for over 3 months, store Antibody #1 and HRP-Conjugated Antibody #2 at -20°C or in a freezer.

Sensitivity

Sample Type	Detection Limit (ng/g or ppb)
Honey	0.2

Erythromycin ELISA Test Kit

Product Description

REAGEN™Erythromycin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Erythromycin in meat(beff,chicken and pork),fish and shrip sampless. The unique features of the kit are:

l High recovery (80-95%),rapid(less than 40 minutes) extraction methods for various samples.

l High sensitivity (0.5ng/g or ppb)and low detection limit (2.5ppb)for fish and shrimp samples.

l A quick ELISA assay(less than 2 hours regardless of number of samples).

l High reproducibility..

Procedure Overview

Kit Contents, Storage and Shelf Life

REAGEN™Erythromycin ELISA Test Kit has the capacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

Kit Contents	Amount	Storage
Erythromycin Coated Plate	1x 96-well Plate(8wells x12 strips)	2-8°C
Erythromycin Standards: Negative control (white cap tube) 0.5ng/mL(yellow cap tube) 1.0 ng/mL(orange cap tube) 2.0 ng/mL (pink cap tube) 4.0 ng/mL(purple cap tube) 8.0 ng/mL (blue cap tube) 100 µg/mL (spiking, optional, red cap tube)	0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL 0.8 mL	2-8°C *
Erythromycin Antibody #1	12 mL	2-8°C *
1X HRP Conjugate Antibody #2	18 mL	2-8°C *
20X Wash Solution	28 mL	2-8°C
Stop Buffer	14 mL	2-8°C
TMB Substrate	12 mL	2-8°C
10X PBSr	25 mL	2-8°C

* If you are not planning to use the kit for over 1 month, store Erythromycin Standards, Erythromycin Antibody and 1X HRP Conjugate at -20°C or in a freezer.

Sensitivity (Detection Limit)

Sample Type	Detection Limit (ppb)
Meat/Fish/shrip	2.5 ppb

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Fumonisin Toxin ELISA Test Kit

Product Description

REAGEN™Fumonisin ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Fumonisin in cereals, dried meat/fish, feed, milk and seed .

The unique features of the kit are:

§ High recovery (>80%),rapid(10-20minutes), cost-effective extraction methods.

§ High sensitivity (1 ng/g or ppb).

§ A quick ELISA assay (less than 1 hours regardless of number of samples).

§ High reproducibility.

Procedure Overview

The method is based on a competitive colorimetric ELISA assay. The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells. The resulting color intensity, after addition of substrate, has an inverse relationship with the target concentration in the sample.

Kit Contents, Storage and Shelf Life

REAGEN™Fumonisin ELISA Test Kit has the Fumonisinacity for 96 determinations or testing of 42 samples in duplicate (assuming 12 wells for standards). Return any unused microwells to the foil bag and reseal them with the desiccant provided in the original package. Store the kit at 2-8°C *. The shelf life is 12 months when the kit is properly stored.

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